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Cell suspension culture as a means to produce polyphenols from date palm (Phoenix dactylifera L.) Ciência e Agrotecnologia
Naik,Poornananda Madhava; Al-Khayri,Jameel Mohammed.
ABSTRACT Date palm accumulates a wide range of secondary metabolites high in nutritional and therapeutic value. In the present study, date palm (Phoenix dactylifera L., cv. Shaishi) shoot-tip-induced callus was used to establish cell suspension cultures in Murashige and Skoog (MS) liquid medium containing 1.5 mg L-1 2-isopentenyladenine (2iP) and 10 mg L-1 naphthaleneacetic acid (NAA). To study the growth kinetics, cultures were maintained for 12 weeks during which weekly measurements were carried out to determine the biomass accumulation based on packed cell volume (%), fresh weight and dry weight (g). In addition, weekly determination of polyphenols (catechin, caffeic acid, kaempferol, and apigenin) was carried out using high performance liquid...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Biomass; Growth curve; Packed cell volume..
Ano: 2018 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-70542018000500464
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Date palm micropropagation: Advances and applications Ciência e Agrotecnologia
Al-Khayri,Jameel Mohammed; Naik,Poornananda Madhava.
ABSTRACT Date palm (Phoenix dactylifera L.) is a fruit tree resilient to adverse climatic conditions predominating in hot arid regions of the Middle East and North Africa. The date fruit contains numerous chemical components that possess high nutritional and medicinal values. Traditional propagation by offshoots is inefficient to satisfy current demands for date palm trees. Alternatively, micropropagation provides an efficient means for large-scale propagation of date palm cultivars. Both somatic embryogenesis and organogenesis, either directly or indirectly though the callus phase, have been demonstrated in date palm in vitro regeneration. Culture initiation commonly utilizes shoot-tip explants isolated from young offshoots. Recently, the immature...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Cryopreservation; Somatic embryogenesis; Somaclonal variation; Organogenesis; Molecular marker..
Ano: 2017 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-70542017000400347
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